Abstract
Author(s): Ahmed J. Mohammed*, Akeel Abo Alard, Balsam G. Hassan
Background: Type 2 Diabetes Mellitus (T2DM) is a chronic metabolic disorder with significant genetic contributions, including Toll-like receptor 4 (TLR4) gene polymorphisms such as Asp299Gly, which may influence disease susceptibility and progression. Objective: This study aims to evaluate the association of TLR4 (Asp299Gly) gene polymorphism with T2DM and its impact on clinical parameters in an Iraqi women. Methods: A case-control study was conducted with 200 female participants (100 women with Type 2 Diabetes Mellitus (T2DM) and 100 healthy controls) aged 35–60 years. Clinical and biochemical parameters, including fasting plasma glucose (FPG), HbA1c, insulin levels, HOMA IR, QUICKI and lipid profiles, were measured. Genotyping for TLR4 (Asp299Gly) gene polymorphism was performed using PCR and restriction enzyme analysis. Results: The AG genotype showed an odds ratio (OR) of 1.33 (P=0.36), while the GG genotype had an OR of 4.51 (P=0.18). The G allele frequency was higher in T2DM patients (20.5%) than controls (15%), with an OR of 1.53 (P=0.1). Clinical parameters did not differ significantly among TLR4 genotypes, except for HDL levels, which were higher in the GG genotype (P=0.04). Conclusion: There was no association between the Toll-like receptor 4 (TLR4) Asp299Gly polymorphism and type 2 diabetes mellitus (T2DM) in the Iraqi women.